Fig. 7: Plasma HRG differentially regulates thrombus formation.
a Immunoblotting of HRG and FXII antigens in equal amounts of plasma from 4 groups of mice as indicated: WT, Hrg−/−, F12−/− and DKO, with transferrin as the loading control. b Tissue factor-induced thrombin generation (TGA) in the plasma from 4 groups of mice was evaluated in the presence of endothelial cells. The peak height (c) and area under the curve (AUC) (d) from individual TGA curve were calculated in these groups as indicated. The number of animals (n value) analyzed in each group was indicated above the bars. e The time to complete arterial occlusion following FeCl3-induced carotid injury were recorded in 4 groups of mice as indicated. The number of animals (n value) analyzed in each group was indicated above the bars. f Representative images of platelet accumulation (Red) and fibrin generation (Green), visualized by Dylight-649-congugated anti-CD42c and Alexa-488-conjugated 59D8 antibody, respectively, at indicated time points following laser injury in the cremaster arterioles in 4 groups of mice as indicated (scale bar: 30 μm) (Supplementary Movie 5). The median fluorescence intensity of platelets (g) and fibrin (h) were calculated and plotted over time. The AUC for platelets (i) and fibrin (j) were analyzed from each individual thrombus in different groups. The number of thrombi (n value) analyzed in each group was indicated above the bars. Data are presented as mean values ± SEM and analyzed by Welch’s ANOVA test (c–e) or Kruskal–Wallis test with Dunn’s multiple comparisons (i and j). Source data are provided as a Source Data file.
