Fig. 2: Characterization of the physical and molecular properties of SynIDPs demonstrate that they are unstructured proteins.
From: Synthetic intrinsically disordered protein fusion tags that enhance protein solubility
A SDS-PAGE of purified SynIDPs. All SynIDPs run slightly higher than their expected molecular weight, which is characteristic of many IDPs. B CD spectra of the SynIDPs shows a random coil structure, which is deduced from the characteristic negative peak at 197 nm and a positive peak at 215 nm. C Kratky plots [I(q)·q2 as a function of q] of the SynIDP reveal a characteristic of flexible disordered chain for SynIDP-1 and SynIDP-2 while demonstrating extended chain conformation for SynIDP-3. D Scattering profiles [I(q) vs. q] of SynIDPs in a log-log plot. Black lines represent Fit of the PEV model to the SAXS data of SynIDP-1 and SynIDP-2. Curves were offset along Y-axis for better visibility. Source data are provided as a Source Data files.
