Fig. 4: Fusion to SynIDPs rescues soluble and functional expression of Z2-LO10 and TEV proteins.
From: Synthetic intrinsically disordered protein fusion tags that enhance protein solubility
A Purified SynIDP-1/2/3-Z2-LO10 after IMAC and SEC. B Log-fold dilutions of SynIDP-Z2-LO10 and SynIDP controls (see Supplementary Fig. 15C) were incubated with CT-2A-EGFRviii, an EGFR positive murine glioma cell line for 48 h and tested for viability by an MTS assay. n = 3 replicates, error bars represent SD. C Purified SynIDP-1/2/3-TEV after His-purification and SEC. D MALDI-TOF MS of products of SynIDP-2-TEV reaction with ELP-tev-FGF21 substrate at 37 °C for 30 min confirms proteolytic activity. The absence of the intact substrate is evident by the lack of a peak at m/z 45,459. The cleaved FGF-21 (m/z of 20,038) and the ELP-tev (m/z of 25,439) can be seen in the MS spectra. The peak at m/z ~ 37,000 matches the expected Mw of SynIDP-2-TEV. Mass spectra for the control and products of SynIDP-1/3-TEV reaction with ELP-tev-FGF21 are shown in SI (see Supplementary Fig. 18). Source data are provided as a Source Data file.
