Fig. 2: S-layer localisation patterns in dividing and non-dividing C. crescentus cells.
From: Cell cycle dependent coordination of surface layer biogenesis in Caulobacter crescentus
Comparison of labelling in (a) non-dividing cells and (b) dividing, stalked cells. Cells were synchronised prior to pulse-chase labelling using SC-mRFP1 and SC-sfGFP as described. The micrographs are taken from a single experimental replicate, of which three were performed with comparable results. Polar labelling can be seen in all cells, but mid-cell labelling is only apparent in dividing cells. Additionally, dual-coloured stalks (SC-sfGFP at the base of the stalk, and SC-mRFP1 at the stalk tip) are indicated by an asterisk. This is consistent with previous research that shows that new stalk material is created from the base of the stalk120. Scale bars = 1 µm.
