Fig. 3: Pause times and fractions of passage at LacI-Os roadblocks under conditions of opposing (red) or assisting (blue) force and with (triangles) or without (circles) GreA.

A The CCDF and (B) characteristic times of all recorded pauses at LacI-Os roadblocks show that the longest pauses were associated with assisting force with and without GreA, followed by shorter pauses with opposing force without GreA, and even shorter pauses with opposing force plus GreA. Note that all distributions except that for opposing force plus GreA include a significant fraction of indefinitely paused ECs. C Passage through LacI-Os roadblocks was more frequent under opposing than assisting force and was enhanced by the addition of GreA. The number of transcription events in each condition are listed above each bar. D The CCDF and (E) characteristic times of all except indefinite pauses at LacI-Os roadblocks show that the longest pauses were associated with opposing force without GreA, followed by shorter pauses with assisting force without GreA, and even shorter pauses with opposing force plus GreA. In (A) and (D), N represents the number of tethered templates that exhibited a transcription event examined under different force and GreA conditions. Data in (B) and (E) represent the exponentially fitted characteristic times ± the 90% confidence intervals (Supplementary Fig. 6).