Fig. 6: CLASP corrects the topology annotation for TMEM126A. | Nature Communications

Fig. 6: CLASP corrects the topology annotation for TMEM126A.

From: Cross-link assisted spatial proteomics to map sub-organelle proteomes and membrane protein topologies

Fig. 6

A Cross-link map of TMEM126A and its interacting proteins. Left: the cross-link map based on the previous annotation; right: the cross-link map based on the CLASP annotation. LMs are shown in green; predicted regions by TMHMM2.0 are shown in purple. IMS-localization of the TMEM126A C-terminus is supported by 1 LM. Since TMEM126A has an even number of TM regions, both termini are likely located in the same sub-compartment, which is confirmed by additional connections to proteins that are not LMs (shown in grey). “Predict region” indicates the protein/protein region, for which a CLASP prediction was made. B Protease protection assay combined with WB to analyze the localization of TMEM126A-HA in HEK293T cells. COXA8-SNAP, which has the same membrane topology as the one CLASP suggested for TMEM126A, serves as a positive control. Experiment was performed once. Source data are provided as a Source Data file.

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