Fig. 1: Rpd3 is sequestered to INQ upon replication stress.
From: Dynamics of DNA damage-induced nuclear inclusions are regulated by SUMOylation of Btn2
a Rpd3 forms inclusions upon acute exposure to 0.05% Methyl methanesulfonate (MMS) which induces replicative stress. Right, representative image; Left, quantification of cells with Rpd3-GFP foci. UNT = untreated. b–d Representative images of colocalization of Rpd3-GFP with Hta2-mCherry, Nic96-RFP and Hos2-mCherry respectively. e Deletions of compartment-specific sequestrases Hsp42 and Btn2 results in a significant reduction of Rpd3 INQ foci in MMS treated cells. f Cellular fractionation supports imaging data showing Hsp42 and Btn2-dependent shift of Rpd3-GFP to the insoluble pellet under stress. W whole cell extract, S supernatant, P insoluble pellet. All error bars represent means ± SEM, n = 3 biologically independent replicates, >100 cells each. ****p < 0.0001, ***p < 0.0002, **p < 0.002, Fisher’s test. Scale bars: 5 µm. Source data are provided as a Source Data file.
