Fig. 2: Sequestration of Rpd3 as a general response to DNA damage.
From: Dynamics of DNA damage-induced nuclear inclusions are regulated by SUMOylation of Btn2
a Rpd3-GFP foci levels following different genotoxic stressors. H2O2 (2 mM), Camptothecin (CPT; 25 uM), Hydroxyurea (HU; 200 mM) and Ethyl methanesulfonate (EMS; 0.5%). Representative images are shown below and quantified above. b Quantification of Rpd3-GFP INQ foci levels in sml1Δ, mec1Δsml1Δ, tel1Δ, and mec1Δsml1Δtel1Δ cells in untreated and MMS treated cells. c Rpd3-GFP foci counts as a function of budding index for unstressed cells harboring sml1, mec1, and tel1 deletions. Mean percentage values of three replicates are shown below each dataset. All error bars represent ±SEM, n = 3 biologically independent replicates, >100 cells each. ****p < 0.0001, Fisher’s test. Scale bars: 5 µm. Source data are provided as a Source Data file.
