Fig. 5: dMP results of XL reaction optimization for R2SP complex. | Nature Communications

Fig. 5: dMP results of XL reaction optimization for R2SP complex.

From: Denaturing mass photometry for rapid optimization of chemical protein-protein cross-linking reactions

Fig. 5

nMP profiles (yellow) and dMP profiles (gray) of R2SP cross-linked with 0, 25, 100, 400 molar excesses of a PhoX, b DSAU, c DSSO, d DSBU, shown as probability densities. Measurement replicates (n = 3) are shown as overlapping curves in shades of yellow or gray. e Quantitative results of R2SP XL optimization at 25, 50, 100, 200, 400 molar excesses of reagents. Bar charts represent the dMP-calculated total inter-XL efficiency (mean ± SD) for each complex and XL condition, from measurement replicates (n = 3). Plain dots represent the specific factor for each complex and XL condition. The black dash line corresponds to the stabilization factor value of 1 indicating a complex abundance similar to the native sample. Source data are provided as a Source Data file.

Back to article page