Fig. 1: The rice near-isogenic lines containing R genes show attenuated endocytosis and increased resistance to rice blast.
From: Phytoalexin sakuranetin attenuates endocytosis and enhances resistance to rice blast
Disease lesion phenotype of M. oryzae on the rice line LTH (a), and the near-isogenic lines IRBLa-A (b), IRBL19-A (c), IRBLta-CP1 (d), IRBL20-IR24 (e), IRBLi-F5 (f), IRBL3-CP4 (g), IRBLk-K (h), IRBLsh-S (i), IRBLKs-S (j) and IRBL9-W (k). All rice lines were inoculated with the M. oryzae strain Guy11. l Quantification of the disease index shown in the images a–k (nLTH = 43, nIRBLa-A = 11, nIRBL19-A = 15, nIRBLta-CP1 = 8, nIRBL20-IR24 = 18, nIRBLi-F5 = 24, nIRBL3-CP4 = 17, nIRBLk-K = 16, nIRBLsh-S = 17, nIRBLKs-S = 19, nIRBL9-W = 27). Root epidermal cells of 6-day old rice LTH (m), and near-isogenic lines IRBLa-A (n), IRBL19-A (o), IRBLta-CP1 (p), IRBL20-IR24 (q), IRBLi-F5 (r), IRBL3-CP4 (s), IRBLk-K (t), IRBLsh-S (u), IRBLKs-S (v) and IRBL9-W(w) were labeled with 4 µM FM4-64 for 90 min. x Quantification of relative fluorescence intensities of plasma membrane versus cytoplasm in rice root epidermal cells shown in the images m–w (nLTH = 194, nIRBLa-A = 127, nIRBL19-A = 121, nIRBLta-CP1 = 103, nIRBL20-IR24 = 129, nIRBLi-F5 = 108, nIRBL3-CP4 = 94, nIRBLk-K = 144, nIRBLsh-S = 126, nIRBLKs-S = 123, nIRBL9-W = 53). LTH Lijiangxintuanheigu. The relative fluorescence intensity is color-coded: red, low; green, medium; and blue, high fluorescence. PM plasma membrane, FM4 = FM4-64. Data are means ± SE; **P < 0.01 (independent-samples two-sided Student’s t test in images l and x). Scale bar = 1 cm in images a–k; Scale bar = 10 μm in images m–w.
