Fig. 4: Resistance to rice blast in the rice chc-cc mutant and Nipponbare lines following treatment with ES9 and ES9-17.
From: Phytoalexin sakuranetin attenuates endocytosis and enhances resistance to rice blast
Leaf phenotypes of rice wild-type Nipponbare (a, d, g) and chc-cc mutant lines #1 and #2 (a). Seedlings were inoculated with spores of the fungus M. oryzae, strain Guy11, and grown for 3 days. The wild-type Nipponbare was also treated with either 50 μM endosidin9 (d) or 50 μM endosidin9-17 (g), or was treated with sterilized water as a control (d, g). b, e Quantification of disease index of the rice shown in image a (nNPB = 46; n#1 = 79; n#2 = 78) and image d (nDMSO = 31; nES9 = 31). h Quantification of lesion length of the rice shown in image g (nDMSO = 64; nES9-17 = 102). c, f, i The expression levels of the MoPot2 gene were assessed (n = 3 biologically independent experiments) in the leaves shown in the images a, d, and g. The actin gene was used as the control gene to check the levels of MoPot2 using real-time PCR. NPB Nipponbare, ES9 endosidin9, ES9-17 endosidin9-17. Data are means ± SE; P values were generated using independent-samples two-sided Student’s t test in images b, e and h and independent-samples two-sided SPSS analysis in images c, f and i. Scale bar = 1 cm.
