Fig. 6: Entry dynamics of OSM-3 and kinesin-II molecules in cilia of mksr-1 mutants and kap-1; mksr-1 double mutants.
From: IFT cargo and motors associate sequentially with IFT trains to enter cilia of C. elegans
a Distance-time plots of OSM-3 mksr-1 (428 tracks from 14 cilia). b Distribution (upper panel) and histogram (lower panel) of docking locations of OSM-3 mksr-1 (average docking location is 428 ± 56 nm). The distribution of docking locations of OSM-3 in wild-type worms is overlayed in black. c Velocity distribution of kinesin-II in wild type (green) and kinesin-II mksr-1 (pink) along the ciliary length. Solid line is the binned average velocity and shaded area indicates the error. d Distance-time plots of OSM-3 kap-1; mksr-1 (173 tracks from 10 cilia). e Distribution (upper panel) and histogram (lower panel) of docking locations of OSM-3 kap-1; mksr-1 (gray; average docking location is 230 ± 56 nm). The distribution of docking locations of OSM-3 in wild-type worms is overlayed in black. f Velocity distribution of OSM-3 in wild type (orange), OSM-3 kap-1 (brown), OSM-3 mksr-1 (light green) and OSM-3 kap-1; mksr-1 (gray) along the ciliary length. Solid line is the binned average velocity and shaded area indicates the error. Average value and error are estimated using bootstrapping (also in c).
