Table 1 Data obtained from tracking single molecule events of different IFT components, entering the phasmid cilia
From: IFT cargo and motors associate sequentially with IFT trains to enter cilia of C. elegans
Worm strain | No. of cilia (No. of worms) | No. of tracks | Moving localizations (Paused localizations) | Docking location (nm) | Measured pause time; \({t}_{{p\_m}}\)(s) (No. of tracks) | Estimated real pause times; \({t}_{{p\_actual}}\)(s) |
|---|---|---|---|---|---|---|
kinesin-II | 12 (7) | 311 | 6693 (225) | 188 ± 20 | 0.9 ± 0.1 (291) | 0.4–0.8 |
OSM-3 | 16 (9) | 419 | 8706 (88) | 334 ± 39 | 0.4 ± 0.1 (415) | 0–0.1 |
IFT-dynein | 14 (7) | 666 | 11,314 (18,561) | 112 ± 8 | 1.8 ± 0.2 (263) | 2.7–8 |
Tubulin | 11 (7) | 170 | 4085 (36) | 660 ± 255 | 0.2 ± 0 (169) | 0 |
OSM-3 kap-1 | 14 (8) | 304 | 8085 (1471) | 315 ± 72 | 0.7 ± 0.1 (286) | 0.4–0.8 |
IFT-dynein kap-1 | 28 (17) | 420 | 4974 (6859) | 189 ± 25 | 1.4 ± 0.3 (261) | 1.5–3.5a |
kinesin-II osm-3 | 20 (10) | 299 | 9490 (603) | 229 ± 30 | 0.7 ± 0.1 (274) | 0.4–0.8 |
OSM-3 mksr-1 | 14 (9) | 429 | 11,672 (116) | 428 ± 56 | 0.3 ± 0 (428) | 0a |
kinesin-II mksr-1 | 12 (9) | 112 | 1741 (148) | 186 ± 29 | 0.7 ± 0.2 (102) | 0.3–0.5a |
OSM-3 kap-1; mksr-1 | 10 (7) | 191 | 3923 (1137) | 230 ± 56 | 0.9 ± 0.2 (173) | 1.1–1.9 |
