Fig. 8: Adoptive transfer of autophagy-activated macrophages (Mφ) alleviates kidney injury in AKI mice.

a Peritoneal Mφ isolated from AKI mice were treated with trehalose, after which the protein levels of LC3 II and P62 were measured. ACTIN was used as the loading control. n = 3 biological replicates for each group, two-way ANOVA with Tukey’s multiple comparison test. b The level of miR-195a-5p in Mφ from AKI mice treated with trehalose. ACTIN was used as the loading control. n = 3 biological replicates for each group, unpaired two-tailed Student’s t test. After endogenous Mφ were deleted by clodronate liposomes (Lipo-Clod), AKI was induced in mice by cisplatin. Then, Mφ from AKI mice (Mφ^AKI) or trehalose-treated Mφ^AKI mice (Mφ^AKI+Tre) were adoptively transferred into the mice at 6 h after cisplatin treatment. c western blot and quantitative analyses of OXPHOS-related proteins. ACTIN was used as the loading control. n = 3 biological replicates for each group, two-way ANOVA with Tukey’s multiple comparison test. d Representative TEM images and hematoxylin-eosin (HE) staining of kidneys from AKI mice after adoptive transfer of Mφ. Scale bars, 2 µm and 50 µm, n = 4 biological replicates for each group, unpaired two-tailed Student’s t test. e Renal function (serum levels of BUN and CREA) detection. n = 6 biological replicates for each group, one-way ANOVA with Tukey’s multiple comparisons test. f Representative micrographs and quantification of TUNEL staining (green) in each group. Scale bars, 20 µm and 50 µm, n = 6 biological replicates for each group, one-way ANOVA with Tukey’s multiple comparisons test. g Schematic representation of the data. Data in (a) and (b) show a representative of three independent experiments. The data are the means ± SEMs. Source data are provided as a Source data file.