Table 6 Primary and secondary antibodies used for Western blot analysis
From: Comparison of intramyocellular lipid metabolism in patients with diabetes and male athletes
Antibody | Type | Company |
|---|---|---|
pAKT Ser 473 | primary | Cell Signalling Technology (London, UK) |
AKT (pan) C67E7 | primary | Cell Signalling Technology (London, UK) |
pAMPK T172 | primary | Cell Signalling Technology (London, UK) |
AMPKα (D63G4) | primary | Cell Signalling Technology (London, UK) |
pIR Tyr 1162 | primary | Thermo Fisher Scientific (Waltham, USA) |
pIRS-1 (Ser 612) | primary | Cell Signalling Technology (London, UK) |
pERK | primary | Cell Signalling Technology (London, UK) |
PS6 | primary | Cell Signalling Technology (London, UK) |
GLUT1 | primary | Proteintech Europe (Manchester, UK) |
GLUT4 | primary | Abcam (Cambridge, UK) |
GAPDH | primary | Proteintech Europe (Manchester, UK) |
Goat anti-rabbit IgG HRP conjugate antibody | secondary for pAKT Ser 473, PS6, pAMPK T172, pERK, pIRS-1 (Ser612), pIR Tyr 1162, GLUT1, GLUT4, AKT (pan) C67E7, AMPKα (D63G4) | Enzo Biochem Inc (New York, USA) |
Anti-mouse IgG, HRP-linked antibody | secondary for GAPDH | Cell Signalling Technology (London, UK) |
