Fig. 2: SPAs enhance massive translation after light exposure. | Nature Communications

Fig. 2: SPAs enhance massive translation after light exposure.

From: The phosphorylation of carboxyl-terminal eIF2α by SPA kinases contributes to enhanced translation efficiency during photomorphogenesis

Fig. 2

a An illustration of the experimental design. Steady-state mRNAs (mRNASS) and polysome-bound (mRNAPL) were isolated in parallel and used for library construction and sequencing for transcriptomic and translatomic analyses, respectively. b Translation efficiency (TE) analysis of 7383 SPA-regulated genes. TE was calculated by normalizing the fold change in mRNAPL with the fold change in mRNASS. Extreme red and green colors indicate ratio 4 and ratio 0 of the fold change, respectively. c Classification of SPA-regulated genes at mRNASS or mRNAPL level in spaQ normalized to the WT. K-means clustering was used to classify the 7383 genes regulated by SPA. Extreme red and green colors indicate 64-fold up-regulation and down-regulation, respectively. Enrichment analysis (Gene Ontology) based on the Fisher Exact test was implemented using the Database for Annotation, Visualization and Integrated Discovery (DAVID) v6.8.

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