Fig. 6: Mutation in SPA1 kinase domain could not rescue the repressed translation efficiency in spaQ after light treatment. | Nature Communications

Fig. 6: Mutation in SPA1 kinase domain could not rescue the repressed translation efficiency in spaQ after light treatment.

From: The phosphorylation of carboxyl-terminal eIF2α by SPA kinases contributes to enhanced translation efficiency during photomorphogenesis

Fig. 6

a The plots of polysome profiling showed different pattern of ribosomal RNA between Col-0, LUC-SPA1/spaQ and LUC-mSPA1/spaQ. Extracted ribosomal RNA were fractionated by 12.5–60% sucrose gradient. The positions of non-polysomal (NP) and polysomal (PL) RNA are indicated on the profiles. b The bar charts showed the percentage of polysomal RNA in total RNA in Col-0, LUC-SPA1/spaQ and LUC-mSPA1/spaQ. Four-day-old etiolated seedlings were incubated either under dark (D) or illuminated 4 h white light (L4h) before sampling. After gradient separation of NP and PL, the RNA was extracted by PCI method. Error bars indicate the mean ± SD (n = 3 biological repeats). The expression of spike-in RNA (Daptomycin) was used for data normalization. Asterisks indicate translation efficiency of conditions statistically different from that of 4-day-old etiolated Col-0 seedlings. *p-value < 0.01, two-sided Student’s t test.

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