Fig. 7: The phosphorylation of C-terminal eIF2α by SPAs promotes translation. | Nature Communications

Fig. 7: The phosphorylation of C-terminal eIF2α by SPAs promotes translation.

From: The phosphorylation of carboxyl-terminal eIF2α by SPA kinases contributes to enhanced translation efficiency during photomorphogenesis

Fig. 7

The plots of polysome profiling showed different patterns of ribosomal RNA between eIF2α.2-WT, eIF2α.2-8A, and eIF2α.2-8D transgenic lines in Col-0 (a) and spaQ (c) backgrounds. Extracted ribosomal RNA were fractionated by 12.5–60% sucrose gradient. The positions of non-polysomal (NP) and polysomal (PL) RNA are indicated on the profiles. Four-day-old etiolated seedlings in Col-0 background (a) and five-day-old infected etiolated seedlings in spaQ background that transiently express eIF2α.2 variants (c) were incubated either under dark (D) or illuminated with 4 h white light (L4h) before sampling. b, d The bar charts show the percentage of polysomal RNA in total RNA in eIF2α.2-WT, eIF2α.2-8A, and eIF2α.2-8D transgenic lines in Col-0 (a) and spaQ (c) backgrounds. Error bars indicate the mean ± SD (n = 3 biological repeats). The expression of spike-in RNA (Daptomycin) was used for data normalization. Asterisks indicate translation efficiency of conditions statistically different from that of eIF2α.2-WT etiolated seedlings under dark condition. *p-value < 0.01, two-sided Student’s t test.

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