Fig. 1: DnaN dynamics over cell cycle.

a A montage of phase contrast and DnaN-sfGFP fluorescence (pseudo-colored as inferno) images for a single representative cell during replication with 2 min. intervals. b Kymograph of time-lapse imaging of the representative cell in a. Intensity profiles are aligned with old poles. Cell boundaries over time are highlighted by dash line. Replication initiation, appearance of early-splitting and late-splitting events are indicated by white, orange and blue arrows respectively in a, b. c Demograph of normalized DnaN-sfGFP fluorescence at different time points of normalized replication duration. d Intensity profiles of the demograph at 30% and 90% replication time in c. Three example profiles for each category are highlighted in blue or pink, with their positions indicated in c. e Frequency of cells that contain two detected DnaN foci (top; n = 1641), and the intensity ratio of bright/dim focus (bottom) as a function of replication time. Dashed line indicates the threshold value of 1.5. f Occurrence of cells with dissimilar ( > 1.5; n = 1092) or similar ( ≤ 1.5; n = 549) intensity of the two DnaN foci as a function of replication time. Source data underlying d–f are provided as a Source Data file. Scale bar in a, c: 1 µm.