Fig. 8: The effect of optogenetic activation of L6CT neurons on S1 activity.
From: Dynamic corticothalamic modulation of the somatosensory thalamocortical circuit during wakefulness
a The effect of L6CT neuron activation on ongoing activity and sensory responses in S1 is measured from recordings of neuronal spiking in S1 while concurrently deflecting a single whisker for somatosensory input and increasing optogenetic activation of L6CT neurons in awake, NTSR1-Cre mice (n = 36 recordings in 11 mice). b Mean waveform +/− standard deviation across S1 neurons identified as either regular spiking (RS) or fast spiking (FS) based on the trough-to-peak time (TPT) of their spike waveform (RS > 0.5 ms and FS < 0.4 ms; see Methods). c Raster plots of an example RS (non-L6CT) neuron’s sensory response with concurrent optogenetic activation of L6CT neurons at 0, 8, and 30 mW/mm². This neuron’s ongoing and stimulus-evoked spiking is suppressed by concurrent optogenetic activation of L6CT neurons (left panel). The right view is an expansion of the same neuron’s sensory response. d. PSTHs of the same neuron’s spiking in c. Note the change in the vertical scale in the right and left views due to differing bin sizes.
