Fig. 4: Binding affinities of FEM1B-EB-EC and its variants towards various C-degrons.
From: Mechanism of Ψ-Pro/C-degron recognition by the CRL2FEM1B ubiquitin ligase
a Sequence alignment of the C-degrons of CCDC89, CUX1, MCRIP1, PSMB5, BEX2 and CDK5R1, with Ψ indicating the specific aromatic residue or His. b ITC curves for the binding of the FEM1B-EB-EC ternary complex to different SUMO fusion C-degrons. c ITC curves for WT FEM1B-EB-EC binding to the CCDC89 C-degron and its variants. d ITC curves for CCDC89 C-degron binding to the FEM1B-EB-EC complex WT and the 5A or 4D mutants. e ITC curves for WT FEM1B-EB-EC binding to the CCDC89 C-degron and its deletion variants. f ITC curves for WT FEM1B-EB-EC binding to the CUX1 C-degron and its variants. g ITC curves for WT FEM1B-EB-EC binding to the PSMB5 C-degron and its variants. h ITC curves for the binding of 23-aa and 10-aa CDK5R1 C-degrons to FEM1B-EB-EC WT and the 5A mutant.
