Fig. 5: Catalytic mechanism of TS. | Nature Communications

Fig. 5: Catalytic mechanism of TS.

From: Discovering a mitochondrion-localized BAHD acyltransferase involved in calystegine biosynthesis and engineering the production of 3β-tigloyloxytropane

Fig. 5: Catalytic mechanism of TS.

A Schematic model of the catalytic pocket that contains 3β-tropanol and tigloyl-CoA. B Comparison of the relative activities of TS and TSH162A using tigloyl-CoA as the acyl donor. C Key residues in the substrate pocket combined with 3β-tropanol. D Comparison of the relative activity of TS and TS mutants (3β-tropanol binding residues mutated to alanine) using tigloyl-CoA as the acyl donor. **P < 0.0001 (TSI35A), **P < 0.0001 (TSQ39A), **P < 0.0001 (TSY280A), **P < 0.0001 (TSN298A), **P < 0.0001 (TSL300A), **P < 0.0001 (TSW340A). E 3β-Tropanol and acyl donors are converted to 3β-tropanol esters via the catalysis of TS. The data are presented as means values ± s.d. Recombinant protein obtained from three independent transformants of TS and each mutant for activity test. Statistical analysis was performed according to the two-sided independent sample t-test.

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