Fig. 7: De novo synthesis of 3β-tigloyloxytropane in N. benthamiana. | Nature Communications

Fig. 7: De novo synthesis of 3β-tigloyloxytropane in N. benthamiana.

From: Discovering a mitochondrion-localized BAHD acyltransferase involved in calystegine biosynthesis and engineering the production of 3β-tigloyloxytropane

Fig. 7: De novo synthesis of 3β-tigloyloxytropane in N. benthamiana.

A Reconstruction of the 3β-tigloyloxytropane biosynthetic pathway in N. benthamiana. B LC-MS detection of target metabolites in tobacco leaves. C The contents of tropinone. D The contents of hygrine. E The contents of 3β-tropanol. F The contents of 3β-tigloyloxytropane. **P = 0.003. G The contents of 3β-acetoxytropane. **P = 0.0068. The control represents tobacco leaves expressing YFP. TS (TSS40T) represents tobacco leaves coexpressing six TA genes and TS (TSS40T). The data are presented as means values ± s.d. Leaves from three independent plants of each line were used for metabolite analysis. Statistical analysis was performed according to the two-sided independent sample t-test.

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