Fig. 8: E. coli bioreactors for the biosynthesis of 3β-tropanol esters. | Nature Communications

Fig. 8: E. coli bioreactors for the biosynthesis of 3β-tropanol esters.

From: Discovering a mitochondrion-localized BAHD acyltransferase involved in calystegine biosynthesis and engineering the production of 3β-tigloyloxytropane

Fig. 8: E. coli bioreactors for the biosynthesis of 3β-tropanol esters.

A PcICS enzymatic assays. B MS data of tigloyl-CoA. C Semi-biosynthetic route of 3β-tigloyloxytropane. D Production of 3β-tigloyloxytropane. Left Y-axis, the content of 3β-tigloyloxytropane. Right Y-axis, the conversion rate from 3β-tropanol to 3β-tigloyloxytropane. HS-CoA means coenzyme A (CoA). Three independent transformants of each engineered E. coli were used in the semi-biosynthesis of 3β-tigloyloxytropane. The data are presented as means values ± s.d.

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