Fig. 1: Structure of PRPF40A tandem WW domains. | Nature Communications

Fig. 1: Structure of PRPF40A tandem WW domains.

From: Intramolecular autoinhibition regulates the selectivity of PRPF40A tandem WW domains for proline-rich motifs

Fig. 1

A Cartoon representation of a representative structure from the calculated ensemble for PRPF40A WW12; the aromatic residues, essential for the folding and ligand interaction, are shown in sticks. B Bar charts representing the intensity ratios for each amine signal in the 1H-15N HSQCs between the paramagnetic and diamagnetic states of the IPSL-labeled proteins; experimental errors are shown as gray bars. Three different positions were selected for labeling: 162, 194, and 202. The green line and the shadow represent the calculated intensity ratios based on the structure of the PRPF40A WW12 and the deviation of the value in the 20-conformer ensemble. Negative values indicate no data for those residues. C Small Angle X-ray Scattering of PRPF40A WW12 protein (black dots indicate scattering intensity values and gray lines the measurement errors), the fitting of the calculated structural ensemble (20 green lines), and the averaged fitting of the ensemble (purple line). Source data are provided as a Source Data file.

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