Fig. 9: Hmg1 SSD mutations do not alter localization of Hmg1 to the A. fumigatus endoplasmic reticulum.

A Colony morphology comparison of the control, Hmg1 parental (hmg1^WT and hmg1^S305P), and Hmg1-GFP strains (hmg1^WT-gfp and hmg1^S305P-gfp) after 5 days growth on minimal media. B Quantitation of colony diameter for the indicated strains at days 1, 2, 3, 4, and 5 post-inoculation. Assays were completed in biological triplicate. C Rosuvastatin and D voriconazole susceptibility assays for the control, Hmg1 parental, and Hmg1-GFP strains. Assays were completed as described in Figure X. Drug concentrations for each well are noted in the included plate diagrams. E Hmg1^WT-GFP and F Hmg1^S305P-GFP localization analyzed by epifluorescence microscopy. Conidia from each strain were inoculated into minimal media, incubated for 12 hrs at 37 °C, and then mounted for microcopy. Images were captured using an exposure time of 600 ms. Note localization to regularly spaced ring structures indicative of A. fumigatus peri-nuclear endoplasmic reticulum. BF = brightfield; UV = ultraviolet. Microscopy experiments were completed three times independently with similar results. Source data are provided as a Source Data file.