Fig. 3: Translation of the model peptides containing Ser and Leu codons by using a cell-free translation system with a hybrid tRNA set. | Nature Communications

Fig. 3: Translation of the model peptides containing Ser and Leu codons by using a cell-free translation system with a hybrid tRNA set.

From: Ser/Leu-swapped cell-free translation system constructed with natural/in vitro transcribed-hybrid tRNA set

Fig. 3: Translation of the model peptides containing Ser and Leu codons by using a cell-free translation system with a hybrid tRNA set.

a The sequence of the model peptides and their coding mRNAs. Xaa and NNN represent the amino acids Ser and Leu, and the codons UCU and CUU, respectively. b MALDI-TOF MS analysis of the model peptides. Peaks corresponding to the full-length model peptides containing either Ser or Leu are labeled with asterisks in the MS spectra. The full MS spectra are shown in Supplementary Fig. 4. The calculated and observed mass of the model peptides are provided in Supplementary Table 1. c Tricine SDS-PAGE analysis of the model peptides. The peptides were analyzed using tricine SDS-PAGE and detected by autoradiography (n = 1). Samples were run on the same gel, and the image was cropped only for the purpose of this figure. Abbreviations: UTR, untranslated region; Std, standard; SL, Ser/Leu-swapped. Source data are provided as a Source Data file.

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