Fig. 5: LC-MS analysis of the products synthesized in a cell-free translation system containing either the natural tRNA extract, the hybrid-SL tRNA set, or the hybrid-SL tRNA set minus one chimeric tRNA. | Nature Communications

Fig. 5: LC-MS analysis of the products synthesized in a cell-free translation system containing either the natural tRNA extract, the hybrid-SL tRNA set, or the hybrid-SL tRNA set minus one chimeric tRNA.

From: Ser/Leu-swapped cell-free translation system constructed with natural/in vitro transcribed-hybrid tRNA set

Fig. 5

a The P2 peptide sequences and their encoding M2 mRNAs are displayed, with NNN denoting the CUU/C and UCU/C codons. The relative abundance of amino acids (Xaa) identified via LC-MS is presented in the pi charts. The relative abundance was calculated from the abundance of each peptide divided by the total abundance of observed peptides. The relative abundance of amino acids is presented in Supplementary Table 3. b The P3 peptide sequences and their encoding M3 mRNAs. The relative abundance of amino acids (Xaa) is presented in the pi charts. The relative abundance of amino acids is presented in Supplementary Table 4. Source data are provided as a Source Data file.

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