Fig. 4: Cryo-EM structure of Nup98FG(298-327) fibrils.
From: Impact of distinct FG nucleoporin repeats on Nup98 self-association
a Cryo-electron micrograph of Nup98FG(298-327) fibrils (1 of the total 11,290 micrographs). b Examples of high resolution 2D classes of the Nup98FG(298-327) fibrils. Scale bar shown on top. c Reconstruction of a Nup98FG(298-327) fibril from low-resolution and big box 2D classes for crossover estimation. d Cross-section of the cryo-EM map of the Nup98FG(298-327) fibril after 3D refinement. Scale bar shown inside. e Single cross-section of the structure of Nup98FG(298-327) fibrils together with the cryo-EM density map (from the top and the side of the fibril axis). Density maps were zoned for clarity, unzoned map is shown in Supplementary Fig. 3.
