Fig. 1: rAAV library screening via intravitreal injection in mice.
From: An adeno-associated virus variant enabling efficient ocular-directed gene delivery across species
a Schematic diagram of rAAV and rAAV variants components. b Three injection routes commonly used for AAV ophthalmic gene therapy. c Mice were sacrificed at D28 and eyes were harvested. The detection of eGFP mRNA expression levels in AAV8 and AAV8 variants after intravitreal injection in mice using RT-qPCR (6 × 108 vg/eye, n = 3). The color depicted represent relative mRNA expression levels from highest to lowest value (red to white, respectively). d The cell types across the retina were illuminated. e Mice were sacrificed at D28 and eyes were harvested. Coronal sections of rAAV-CBA-eGFP transduced mice retina. Immunofluorescence (IF) stained sections (Green) with antibodies against eGFP indicate the positively transduced cell type across the retina. (Scale bar: 50 μm, GCL ganglion cell layer, INL inner nuclear layer, ONL outer nuclear layer;). We conducted three repetitions for each sample, with each assay being operated independently. Source data are provided as a Source Data file.
