Fig. 1: Scheme of in vitro co-transcriptional translation assay and quantification.
From: 5′UTR G-quadruplex structure enhances translation in size dependent manner
a Schematic of the co-transcriptional translation assay. The DNA construct features a T7 promoter (yellow), E. coli ribosome binding site (RBS in orange) and GFP gene. b, c Example curves of transcription and translation data. The blue curve exemplifies the transcription readout in real-time via a Cy3 molecular beacon that fluoresces when bound to mRNA. The green curve represents translation readout as the fluorescence of sfGFP. The initial linear phases of curves are used to quantify the transcription and translation initiation rate, respectively. The calculation is described in “Method”.
