Fig. 2: scUTRquant-derived gene and 3′UTR expression is precise and accurate.
From: Quantifying 3′UTR length from scRNA-seq data reveals changes independent of gene expression
a Correlation of unique molecular identifiers (UMIs) per gene obtained by scUTRquant and CellRanger for a mouse heart 10x Genomics demonstration dataset. Inset: Boxplot showing median and IQR of Spearman’s ρ for six mouse 10x Genomics demonstration datasets. Whiskers indicate 1.5*IQR. b Correlation of 3′UTR isoform counts obtained from FACS-sorted HSCs comparing counts from bulk 3′ end sequencing methods with scUTRquant analysis of scRNA-seq data. Inset: Spearman correlations from all (N = 12) pairs of HSC bulk and scRNA-seq replicates, shown as in (a). c Correlation of 3′UTR isoform counts between two replicates of bulk 3′ end sequencing for FACS-sorted HSCs is shown. Inset: Spearman correlations from all (N = 6) pairs of HSC bulk sequencing replicates, shown as in (a). d Correlation of scUTRquant 3′UTR isoform counts between two replicates of scRNA-seq of FACS-sorted HSCs. Inset: Spearman correlations from all (N = 3) pairs of HSC scRNA-seq replicates.
