Fig. 3: Reprogrammation, derivation, and CNS cells differentiation.
From: Herpes simplex encephalitis due to a mutation in an E3 ubiquitin ligase
A Schematic diagram of the reprogrammation and differentiation protocols used. B Immunocytochemistry analysis of iPSCs colonies revealed the expession of pluripotent markers OCT4, TRA-1-60, and SOX2. C Immunocytochemistry analysis of the ability of iPSCs-derived embryonic bodies to differentiate into the 3 germ layers. PAX6, SMA, and AFP are markers for ectoderm, mesoderm, and endoderm respectively. Results represent the analysis of 1 iPSCs colony and is representative of results obtained for all iPSCs colonies (n = 2 per donor). D Characterization of neurons. Compared to neuronal precursors, neurons exhibit increased expression of typical neuronal markers assessed by qPCR. E Characterization of astrocytes. Compared to neuronal precursors, astrocytes exhibit increased expression of typical astrocyte markers, assessed by qPCR.
