Fig. 5: WWP2-dependent HSV-1 genomes number in iPSCs-derived neural stem cells (NSCs).
From: Herpes simplex encephalitis due to a mutation in an E3 ubiquitin ligase
Quantification by polymerase chain reaction of the number of viral genome in neural precursor cells (A), neurons (B) and astrocytes (C) from WWP2 p.R841 individuals [black, N = 5 (A), N = 6 (B), N = 3 (C)] and from WWP2 p.R841H individuals [red, N = 8 (A), N = 9 (B), N = 8 (C)] without or with an IFNα2b pretreatment (+IFN). Results represent the mean ± standard error of 1 experiment among 2 (A) and 1 (B, C). Statistical analyses were performed using an unpaired two-tailed Student t-test. A *, ** mean for P = 0.0028 and P < 0.0001. B *, ** mean for P = 0.0046 and P = 0.0001. The purple triangle and the grey square correspond to patient with HSE and to patient’s father respectively.
