Fig. 6: Intracerebral inoculation of BAP-like amyloids enhances α-Syn pathophysiology in C57BL6/C3H F1 mice.

a Motor function assessed by quantifying time to turn around, wirehang and riding time. Blue bars, mice injected with rBapB-PFF. N = 12. Two tailed unpaired t test, *p = 0.031, **p = 0.0023, *p = 0.038, *p = 0.0177, **p = 0.005, *p = 0.049, *p = 0.0178. Bars represent the means and standard errors of means. Motor data are compiled from two independent cohorts. b Anterior striatal TH innervation was quantified by optical density. Ø, N = 5; rBap-PFF, N = 11. **p = 0.072. c Stereological quantification of TH-immunoreactive neurons in the SNpc. Ø, N = 7; rBap-PFF, N = 11. *p = 0.0462. d Striatal NeuN was quantified by optical density. Ø, N = 5; rBap-PFF, N = 7. *p = 0.0177. e Images for TH staining in posterior striatum. f TH staining of dopaminergic neurons in coronal sections of the SNpc. g Representative images of intraneuronal phospho-α-Syn inclusions in the ipsilateral (IL) SNpc of rBapB-PFF injected mice. h Quantification of Ser129 phospho-α-Syn densitometry in the contralateral (CL) and IL of midbrain. Ø, N = 6; rBapB-PFF, N = 10. **p = 0.047, *p = 0.011. Data were analyzed using two-tailed Mann–Whitney test (b–d, h). i mRNA level of α-syn, hsc70 and lamp2A genes. N = 5, **p = 0.0041. j Western-blots analyzing α-Syn and LAMP-2A expression. k Quantification of total α-Syn and LAMP2A levels normalized to beta-actin. N = 5, *p = 0.0476, **p = 0.004. Data were analyzed using one-tailed Mann–Whitney test (i, k). l Average of the α-Syn half-life. Disappearance of α-Syn was quantified as the mean of three experiments. *p = 0.0273. m Representative images of α-Syn turnover during 48 h. n Western-blots analyzing α-Syn and LAMP-2A expression in SH-SY5Y. o Quantification of total α-Syn and LAMP2A levels normalized to beta-actin. N = 3, p = 0.05. Data were analyzed using Kruskal–Wallis with Dunn’s multiple comparison test (l, o). For all panels, data are shown as means, and error bars are the SE of means. *p < 0.05, **p < 0.01, ***p < 0.001. Mice intrastriatally injected with rBapB-PFF (red bars), monomeric rBapB-MON (grey bars) or PBS (white bars). MW; molecular weight. Source data are provided as a Source Data file.