Fig. 6: aiEC heterogeneity changes during reprogramming.
From: Direct conversion of cardiac fibroblasts into endothelial-like cells using Sox17 and Erg
a UMAP of aiEC scRNAseq labeled by sample type. b UMAP of aiEC scRNAseq labeled by cluster name. c Percent cluster composition of samples. Top three aiEC-related cluster percentages are labeled for SEG samples. d, Expression profiles of fibroblast and endothelial genes. e Top 10 marker gene heatmap for each cluster. f Cluster-specific expression of reprogramming and endothelial genes. g Heatmap of top 10 identified gene regulatory network regulons for selected aiEC endothelial-like clusters. h Expression profile of Elk3. i Venn diagram of aiEC conserved marker genes and Elk3 regulon genes. j Venn diagram of Elk3 regulon genes, H3K27ac SEG upregulated genes in neonatal iECs and H3K27ac neonatal heart endothelial cell genes. k GO Biological Process Enrichment analysis of Elk3 regulon to H3K27ac HEC and SEG, and aiEC conserved genes (one-sided Fisher’s exact test with Benjamini–Hochberg correction).
