Fig. 7: SFC potentiates BF-LHb glutamatergic functional connection.
From: The basal forebrain to lateral habenula circuitry mediates social behavioral maladaptation
a Schematic illustration of brain slice recordings in BF vGluT2 neurons. b Representative membrane potential traces of active (top) and silent (bottom) neurons from unconditioned (left) and conditioned (right) mice. The numerical voltage at the right of each trace indicates resting membrane potential. c Proportion of active and silent neurons. Unconditioned, n = 18 neurons from 3 mice; conditioned, n = 19 neurons from 4 mice. d Representative traces of positive (60 pA, top) and negative (−30 pA, bottom) current injections in BF vGluT2 neurons from an unconditioned (left) and a conditioned (right) mouse. e Comparison of membrane resistance between unconditioned and conditioned groups. Unconditioned, n = 16 neurons from 3 mice; conditioned, n = 18 neurons from 4 mice. P = 0.9973, two-sided unpaired t-test. f The number of action potentials in response to various current injections. Unconditioned, n = 18 neurons from 3 mice; conditioned, n = 19 neurons from 4 mice. g Schematic illustration of optogenetic activation of axon terminals of BF vGluT2 neurons and patch-clamp recording in LHb neurons. h Representative traces of postsynaptic AMPA (bottom) and NMDA (top) currents from an unconditioned (left) and a conditioned (right) mouse. The black dots on traces indicate the amplitudes of AMPA and NMDA currents. i Quantification of AMPA/NMDA ratio in both groups. Unconditioned, n = 10 neurons from 4 mice; conditioned, n = 8 neurons from 4 mice. P = 0.0138, two-sided unpaired t-test. j Representative traces of postsynaptic currents from an unconditioned (gray) and a conditioned mouse (red) elicited by 20 Hz (left) and 10 Hz (right) paired-pulse light stimuli. k Comparison of paired-pulse ratio (PPR) of postsynaptic currents between two groups. 50 ms: unconditioned, n = 8 neurons from 3 mice; conditioned, n = 11 neurons from 3 mice; P = 0.0092. 100 ms: unconditioned, n = 12 neurons from 3 mice; conditioned, n = 12 neurons from 4 mice; P = 0.0300, two-sided unpaired t test. Error bars indicate mean ± SEM. *P < 0.05, **P < 0.01. Source data are provided as a Source Data file.
