Fig. 4: TRF1 release from Tcea1–/– telomeres is associated with telomere dysfunction induced foci (TIFs).
From: Transcription stress at telomeres leads to cytosolic DNA release and paracrine senescence
A Immunofluorescence of 53BP1 and γH2AX with in situ hybridization of telomeric DNA (TelC) in wt and Tcea1–/– MEFs. Arrows denote TIFs on telomeres. The graph depicts the mean percentage of cells presenting ≥5 TIFs (n = 3). B pATM protein levels in wt and Tcea1-/- MEFs whole-cell extracts (n = 3). The graph depicts the total ATM-normalized protein expression levels (n.e.l.). C TRF1 protein levels in whole-cell extracts from wt and Tcea1–/– MEFs. Fibrillarin was used to normalize protein expression levels (n.e.l., n = 4). D Trf1 mRNA levels in wt and Tcea1-/- MEFs (n = 3). E ChIP signals of TRF1 protein (shown as percentage of input after IgG normalization) on telomeres of wt and Tcea1-/- MEFs (n = 5). F Immunofluorescence of TRF1 with in situ hybridization of telomeric DNA (TelC) in wt and Tcea1–/– MEFs. The graph depicts the mean fluorescence intensity (MFI) of TelC in Tcea1–/– MEFs and wt controls (n = 4). G OxiDIP signals of 8-oxoG (shown as percentage of input after IgM normalization) on telomeres, GC-rich and AT-rich regions of untreated wt, H2O2-treated wt and untreated Tcea1–/– MEFs (n = 3). H Immunofluorescence against 8-oxoG with in situ hybridization of telomeric DNA (TelC) in wt and Tcea1–/– MEFs. White arrows indicate 8-oxoG signal on telomeres (n = 4). The graph depicts the 8-oxoG mean fluorescence intensity (MFI) on telomeric DNA (TelC) in Tcea1–/– and wt MEFs. Data analysis was performed using two-tailed Student’s t test. All data are presented as mean values ± SEM. Unless otherwise indicated, n = biologically independent experiments and scale bars are set at 5μm. Source data are provided as a Source Data file.
