Fig. 5: BsaP structure prediction, Fe–S cluster formation, and phylogenetic analyses.
From: Mycobacterial biotin synthases require an auxiliary protein to convert dethiobiotin into biotin
a Comparison of the crystal structure of a [3Fe–4S] + ferredoxin (4ID8, left) with the AF prediction for BsaP.tb (right). Impact on Fe–S cluster reconstitution on the UV-spectra of BioB.tb and BsaP.tb. The dashed lines represent 420 nm and 452 nm, respectively (b) and the enzymatic activity of BioB.tb (c). #Indicates Fe–S cluster reconstitution with Fe (II/III) ammonium sulfate and Na2S. [BioB + BasP]* was reconstituted as a mixture of both proteins. d Consensus maximum log-likelihood phylogenetic tree of BsaP homologs. To the right of the tree, a CDS multiple sequence alignment for the BsaP homologs is shown, where aligned portions are drawn in gray, and alignment gaps are depicted by a line. The BsaP consensus sequence is shown for the region containing the conserved cysteine residues (Cys44, Cys47, and Cys64 of BsaP.tb), which are highlighted in orange. c n = 3. Two-sided unpaired t test; ***p = 0.0004, **p = 0.0141; ns p > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001. Error bars, mean ± SD. b, c Source data are provided as a Source data file.
