Fig. 2: Association of ASRGL1 loss and TDP-43 proteinopathy in brain samples of ALS patients.
From: TDP-43 proteinopathy in ALS is triggered by loss of ASRGL1 and associated with HML-2 expression
a–f FFPE brain cortex samples from 3 pre-selected ALS individuals with very low levels of ASRGL1 and 3 normal controls were stained by immunofluorescence with ASRGL1 and TDP-43 antibodies. a Representative immunofluorescence images of brain cortex showing ASRGL1 and TDP-43 in an ALS individual and a normal control. b Percentage of neurons positive for ASRGL1 in normal controls and ALS brain samples. c Percentage of neurons showing cytoplasmic TDP-43. d Percentage of glial cells positive for ASRGL1 in controls and ALS patients. e Percentage of glial cells showing cytoplasmic TDP-43. Data in (b–e) analyzed by two-sided Unpaired T-test and represented as mean ± SEM. f Amplified image of brain cells of the same cortical brain sample of an ALS patient showing nuclear TDP-43 staining associated with perinuclear staining of ASRGL1 (upper panels) and loss of ASRGL1 associated with cytoplasmic TDP-43 (lower panels). Source data are provided as a Source Data file.
