Fig. 1: Detrimental activity of P. brassicacearum R401 is facilitated by salt stress in soil.
a log2-transformed shoot fresh weight of A. thaliana plants grown axenically on ½ MS agar plates for 19 dpi. At 14 dpi plants were flushed with either heat-killed (HK) or live wild-type (WT) R401 cells. Five minutes after flushing plants were transferred to new sterile plates and grown for five days (n = 5 plants). b log2-transformed shoot fresh weight of A. thaliana plants grown in the gnotobiotic Flowpot system for 28 dpi in the presence or absence of 100 mM NaCl and either heat-killed (HK) or live wild-type (WT) R401 cells (n = 30 plants). c log2-transformed shoot fresh weight of A. thaliana plants grown in the non-sterile Cologne agricultural soil (CAS) in the greenhouse for 28 dpi in the presence or absence of 100 mM NaCl and either heat-killed (HK) or live wild-type (WT) R401 (n = 16 plants). a–c Representative images illustrating the respective plant phenotypes are shown below each plot. Within each figure panel, all images are to scale. Statistical significance was determined by Kruskal-Wallis followed by Dunn’s post-hoc test and Benjamini-Hochberg adjustment. Significance compared to HK is indicated by black asterisks (∗∗ and ∗∗∗ indicate p < 0.01, and 0.001, respectively; ns, not significant). Statistical comparisons were conducted between WT and HK samples for each NaCl treatment separately. Boxplots show 25th percentile, median, and 75th percentile.
