Fig. 1: Adam9 KO mice are highly susceptible to EMCV infection of the heart.
A WT (n = 9) and Adam9 KO (n = 6) mice were infected by intraperitoneal (i.p.) injection of 105 PFU of EMCV and survival was assessed. Adam9 KO mice demonstrated accelerated mortality compared to WT mice in response to EMCV infection (***P = 0.0002, Mantel–Cox survival analysis). Survival data represents two independent experiments. WT and Adam9 KO mice were infected i.p. with 105 PFU of EMCV and EMCV titers were measured by plaque assay at 6, 24, and 48 h p.i. in serum (B) and hearts (C). B EMCV titers in the serum were delayed in Adam9 KO compared to WT mice at 6 h pi. (**P = 0.0064) but were higher in Adam9 KO compared to WT mice by 48 h p.i. (**P = 0.0027) by a two-tailed, unpaired t-test. C In addition, EMCV titers in the heart were also higher in Adam9 KO compared to WT mice at 24 h p.i. (*P = 0.0318) and 48 h p.i. (**P = 0.0046) by a two-tailed, unpaired t-test. B, C All data are representative of three independent experiments with similar results. Each symbol represents an individual mouse (n = 6 for 6 h p.i.; n = 7 for 24 h p.i.; n = 6 for WT and n = 8 for KO for 48 h p.i.), and small horizontal lines indicate the mean. Source data are provided in the Source Data file.
