Fig. 2: Dynamic phosphorylation of amino acids using simple fuels.
From: Active droplets through enzyme-free, dynamic phosphorylation
a The hydrolysis of the phosphorylating agent MAP. b The amino acid-based catalysts were tested to convert the phosphorylating agent MAP. *pH 10.5 of Tyr containing sample due to low solubility at pH <pKa. c The phosphorylating agents tested to phosphorylate histidine. **Not suitable due to many side reactions. d The concentration profiles as a function of time. The markers represent data determined by NMR, and the solid line represents a kinetic model. e The reaction network of the cycle with 1-, 3-pHis, and 1,3-bpHis as observed phosphorylated species. All experiments were performed in triplicate, and the conditions used were 75 mM amino acid, 80 mM of MAP, 50 mM DAP or 100 mM TMP in a 500 mM MES buffered solution at pH 6.5.
