Table 1 Apparent dissociation constant (Kd) values for the interaction of 8–17 DNAzyme variants with different divalent ions derived from global folding and catalytic activity measurements

From: The 8-17 DNAzyme can operate in a single active structure regardless of metal ion cofactor

Metal ion

DNAzyme construct

Kd of metal:DNA interaction estimated using CD spectroscopy

Kd of metal:DNA interaction estimated using activity assays

Zn2+

8–17_short

15 µM

4 mM

crystallographic

23 µM

3 mM

Mg2+

8–17_short

94 µM

14 mM

crystallographic

90 µM

9 mM

Pb2+

8–17_short

<100 µMa

̴ 0.1 mM

crystallographic

41 µM

̴ 0.2 mM

  1. aPb2+ affinity to 8–17_short is impossible to accurately derive from the CD titration, as a second structural transition commences before the first one is saturated (see text). The estimate given in the table is based on the first few datapoints in CD titration, assuming titration endpoint being \({\Delta \varepsilon }_{280{nm}}\approx 0\), similar to other metals.
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