Fig. 7: Experimental set-up to use ex vivo specimens to validate cervix-optimized DBSI parameters. | Nature Communications

Fig. 7: Experimental set-up to use ex vivo specimens to validate cervix-optimized DBSI parameters.

From: Whole cervix imaging of collagen, muscle, and cellularity in term and preterm pregnancy

Fig. 7: Experimental set-up to use ex vivo specimens to validate cervix-optimized DBSI parameters.The alternative text for this image may have been generated using AI.

a A specimen (3 mm thickness) was dissected from the posterior midline of fresh total hysterectomy uteruses. Labels “IOS” and “ECC” indicate internal OS of cervix and endocervical canal respectively. b The specimen was embedded in 2% agar gel and imaged with a Varian 11.7 T MRI, using DBSI sequence. DBSI maps were computed by in-house cervix-optimized DBSI software. c After MRI, the specimen was fixed in formalin, transferred, and embedded in a paraffin block along the plane of MR imaging (orange dotted lines indicates the same plane). d The stained slide-mounted 5 µm histologic sections were digitized and converted to histology maps. The histology maps were then registered to the DBSI maps.

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