Fig. 2: The DBR1 knockout cell line C22 is 20-fold less effective in lariat turnover.
From: The debranching enzyme Dbr1 regulates lariat turnover and intron splicing
A The lariat read recovery rate (lariat reads/total mapped reads) in C19, C22 and two 293T control samples. B Fold change between DBR1 KO and wild type samples in the coverage of individual introns that were classified by ShapeShifter11 as exhibiting lariat accumulation. C Sequence logo of branchsites from lariat reads recovered in 293T and C22 samples from introns with a single recovered branchpoint. D Sequence logo of the top 100 5’ splice sites ranked by lariat read counts in 293T and C22 samples. E Change in lariat levels between C22 and 293T samples for annotated U12 introns as well as U2 introns from the genes containing U12 introns. F Tally of branchpoints reported in previous mapping studies (Pineda13 and Mercer10) and those found in DBR1 KO cell lines. G Branchpoint nucleotide composition of lariats reads recovered in 293T and C22 samples. H Distribution of the maximum branchpoint functional score in an 11 bp window centered on branchpoints from lariat reads recovered in 293T (n = 1374 branchpoints) and C22 samples (n = 15829 branchpoints; p value from two-sided t-test; center line represents median; lower and upper bounds of the box represent the 25th and 75th percentile, respectively; lower and upper whiskers extend to the smallest or largest value no further than 1.5x the inter-quartile range from the 25th or 75th percentile value, respectively; outliers not shown). Source data are provided as a Source Data file.
