Fig. 4: AQR recruits Dbr1 to branchpoints.
From: The debranching enzyme Dbr1 regulates lariat turnover and intron splicing
A For each Dbr1-interacting RNA-binding protein, the change in normalized lariat levels between C22 and 293T samples for introns with and without reported eCLIP binding sites (mean ± SE shown; n = 15087 introns for AQR, 230 introns for HNRNPA1, 2462 introns for HNRNPC, 4322 introns for HNRNPL, 6330 introns for PTBP1, 2576 introns for RBM22 and 280 introns for SRSF7; p-values from two-sided t-test). B For the three RBPs with significant changes in (A), the change in lariat levels between C22 and 293T samples is compared to the location of RBP binding sites relative to the 5’ splice site and branchpoint. C Reciprocal co-IP of Dbr1 and AQR in 293T cells (top; n = 3 independent replicates), and Dbr1 and AQR levels in untreated (UT), non-targeted control (NC) and two AQR-targeted siRNA knockdown samples (KD1 and KD2, bottom; n = 3 independent replicates). D Lariat mapping of RNA-seq data from control and AQR knockdown samples (n = 3 independent replicates shown in color with mean ± SE in black). E Branchpoint nucleotide composition of lariat reads recovered in control and AQR knockdown samples. F Fold-change in lariat levels between C22 and 293T samples for introns containing the AQR binding motif learned from eCLIP peak sequences (mean ± SE shown; n = 17304 introns for both motif and no motif categories). Source data are provided as a Source Data file.
