Fig. 4: Loss of transcription reduces the amount of DNA in euchromatic phase. | Nature Communications

Fig. 4: Loss of transcription reduces the amount of DNA in euchromatic phase.

From: Active transcription and epigenetic reactions synergistically regulate meso-scale genomic organization

Fig. 4: Loss of transcription reduces the amount of DNA in euchromatic phase.

a Grayscale heatmap density rendering of super-resolution images of DNA in control (DMSO, left panel, n = 19 nuclei) and actinomycin D (ActD, right panel, n = 20 nuclei) treated HeLa nuclei. All scale bars − 3 μm. b Zoomed in views of DMSO and ActD treated nuclei. Boxes shown in a are zoomed into. All scale bars - 1 μm. c Along the blue (DMSO) and red (ActD) line segments, we plot the chromatin heatmap intensity (corresponding to the total DNA content) for the DMSO-treated control nucleus (in blue) and ActD-treated nucleus (in red). The DMSO-treated nucleus shows a wider distribution of small heterochromatin domains, while the ActD treated nucleus shows a greater compaction with isolated large heterochromatin domains. d Numerical prediction of distribution of total DNA (in grayscale) in a nucleus with (DMSO) and without (ActD) transcription mediated chromatin extrusion. e Distribution of total DNA content along the blue (red) line in d under DMSO (ActD) treatment. The black dashed line shows the level of total DNA predicted in the euchromatin phase of DMSO and ActD treated nuclei.

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