Fig. 2: p27 promotes STAT3 binding enrichment on chromatin. | Nature Communications

Fig. 2: p27 promotes STAT3 binding enrichment on chromatin.

From: C-terminally phosphorylated p27 activates self-renewal driver genes to program cancer stem cell expansion, mammary hyperplasia and cancer

Fig. 2

a Venn diagrams of p27 and STAT3 binding peaks in 1833 and 1833shp27 cells. b Localization of p27 and STAT3 peaks. c p27 and STAT3 DNA binding heatmaps show effects of p27 depletion on STAT3 binding in 1833 and 1833shp27 cells. d Venn diagrams show how p27 knockdown affects numbers of target genes bound by STAT3 and/or p27 at promoter sites ±5 kb from TSS in 1833 and 1833shp27 cells. e DNA binding heatmaps for p27 and STAT3 binding +/−5 kb from Transcription Start Site (TSS) of 4001 co-target genes in 1833 and 1833shp27 cells. f Enrichment of each of p27 and STAT3 at the 4001 p27/STAT3 co-target genes (+/−5 kb TSS) is reduced by p27 knockdown. g Pathway analysis of STAT3/p27 co-target genes that have p27-dependent STAT3 binding to their promoters. h Top binding motifs at sites of STAT3 binding and for regions bound by STAT3, p27, and cJun. i Venn diagram shows numbers of target genes bound by STAT3, cJun, and p27 at +/−5 kb of their TSS. p-values were represented by unpaired two-tailed T Test. Source data are provided as a Source data file.

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