Fig. 3: p27 is co-recruited to chromatin with STAT3, cJun, and CBP to induce stem cell-driver gene expression. | Nature Communications

Fig. 3: p27 is co-recruited to chromatin with STAT3, cJun, and CBP to induce stem cell-driver gene expression.

From: C-terminally phosphorylated p27 activates self-renewal driver genes to program cancer stem cell expansion, mammary hyperplasia and cancer

Fig. 3

a Venn diagram shows p27-upregulated genes. b Gene expression heatmaps of p27-upregulated genes. c Schematic of p27-activated genes with p27/STAT3/cJun colocalization at their promoter. d p27, STAT3 and cJun binding enrichment at the promoters (+/−5 kb of Transcription Start Site (TSS)) of 96 p27/STAT3/cJun bound p27-activated genes in 1833 and after p27 depletion. p-values were represented by unpaired two-tailed T Test. e Pathway analysis of 96 p27/STAT3/cJun co-targets that are p27-upregulated. f p27, STAT3, and cJun co-occupied peaks at MYC promoter. g KM plot shows reduced relapse-free breast cancer survival with increased MYC expression p = 0.0017, n = 3951 cancers. h–l ChIP-qPCR shows p27 (h); STAT3 (i); cJun (j) and CBP (k) binding, and H3K27 acetylation (l) at the MYC promoter. m Immunoprecipitation (IP) shows p27CK-DD-dependent p27, STAT3, and CBP association. All graphs show mean ± SEM from N = 3 biological replicate assays. p-values were represented by paired one-tailed Student’s T Test. p values are shown in graphs. Source data are provided as a Source data file. ESC embryonic stem cell.

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